This book explores the groundbreaking integration of CRISPR technology into the field of clinical chemistry, presenting a transformative approach to the ultra-sensitive detection of circulating disease biomarkers. It highlights how conventional diagnostic techniques often fall short in identifying low-abundance biomarkers essential for early and accurate disease detection. By leveraging the precision and versatility of CRISPR-associated enzymes, the text demonstrates how rapid, cost-effective, and highly specific assays can be developed for diverse clinical applications. The chapters cover fundamental CRISPR mechanisms, the role of clinical chemistry in diagnostics, and advanced detection strategies that surpass the limitations of traditional methods. Detailed case studies illustrate practical applications, while discussions on challenges and future directions provide valuable insights for ongoing research. Emphasis is placed on the role of CRISPR in advancing personalized medicine, enabling treatments tailored to individual biomarker profiles. The book also examines the ethical, technical, and clinical considerations of deploying CRISPR-based tools in healthcare. With contributions from international experts, it serves as a comprehensive reference for researchers, clinicians, and students in chemistry, biotechnology, and medical sciences. Ultimately, this work underscores the paradigm shift CRISPR brings to diagnostic science, paving the way for earlier intervention, improved patient outcomes, and a new era of precision healthcare.